Systems for producing cellular immunotherapeutics and methods of use thereof

A system for producing an immunotherapeutic product, the system comprising:

• a first module configured for processing cellular material associated with a disease of a patient, the first module comprising a fluid inlet port and a separate fluid outlet port;
• a second module configured for generating immune cells, the second module comprising a cell culture chamber, a fluid inlet port, and a separate fluid outlet port disposed on opposite sides of the cell culture chamber, the separate fluid outlet port positioned at a height above the fluid inlet port, wherein the cell culture chamber comprises a monocyte-binding substrate, and wherein the fluid inlet port and the separate fluid outlet port are fluidically coupled to the cell culture chamber and configured to provide flow of a liquid culture medium across the monocyte-binding substrate from the fluid inlet port to the separate fluid outlet port that minimizes mechanical detachment of cells from the monocyte-binding substrate;
• and
• a third module separately coupled to and in fluidic communication with each of the first and second modules, the third module comprising a first inlet port for fluidic communication with the first module and a separate second inlet port for fluidic communication with the second module such that the third module separately receives a flow of processed cellular material from the first module and separately receives a flow of immune cells from the second module, wherein the third module is configured to co-culture the processed cellular material and the immune cells to produce an immunotherapeutic product.