Multispecific proteins
AssigneeMolecular Partners AG
InventorsNicolo Rigamonti, Clara Domke, Valérie Perrine Calabro, Pamela Anna Trail Smith, Victor Levitsky, Niina Elisabet Veitonmäki
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Abstract
The present disclosure relates to multispecific proteins comprising designed ankyrin repeat domains with binding specificity for different targets, such as, e.g. CD40 and FAP. In addition, the present disclosure relates to nucleic acids encoding such multispecific proteins, pharmaceutical compositions comprising such multispecific proteins or nucleic acids, and the use of such binding proteins, nucleic acids or pharmaceutical compositions in methods for treating or diagnosing diseases, such as cancer, in a mammal, including a human.
Claims19
A recombinant protein comprising:
- a first ankyrin repeat domain that specifically binds serum albumin;
- a second ankyrin repeat domain that specifically binds fibroblast activation protein (FAP);
- a third ankyrin repeat domain that specifically binds CD40;
- and
- a fourth ankyrin repeat domain that specifically binds CD40;
- wherein said ankyrin repeat domains are arranged, from the N-terminus to the C-terminus, according to the following formula:
- (serum albumin binding domain)—(FAP-binding domain)—(CD40 binding domain)—(CD40 binding domain);
- wherein said serum albumin binding domain comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1;
- wherein said FAP binding domain comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2;
- and
- wherein each of said CD40 binding domains independently comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 3.
A recombinant protein comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 5, wherein said recombinant protein binds human FAP, human CD40, and human serum albumin with a KD value of or below 100 nM.
A recombinant protein comprising the amino acid sequence of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.