Site-specific serine ADP-ribosylated proteins and peptides and method for producing the same
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsJuan José Bonfiglio, Ivan Matic
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Abstract
The present invention relates to a method for producing a site -specifically serine ADP-ribosylated protein or peptide being, comprising: (a) subjecting a protein or peptide comprising or consisting of an amino acid sequence comprising two or more serines, wherein at least one serine is phosphorylated and at least one serine is non-phosphorylated, to a serine ADP-ribosylation reaction. The present invention also relates to a site-specifically serine ADP-ribosylated protein or peptide produced by the method of the invention.
Claims16
A method for producing a site-specifically serine Adenosine Diphosphate (ADP)-ribosylated protein or peptide, comprising:
- (a) subjecting to a serine ADP-ribosylation reaction a protein or peptide comprising an amino acid sequence having two or more serines, wherein at least one specified serine is phosphorylated to contain one or more phosphate groups, and at least one specified serine is non-phosphorylated,
- wherein the at least one specified non-phosphorylated serine becomes ADP-ribosylated and the at least one specified phosphorylated serine is protected from serine ADP-ribosylation, thereby obtaining a site-specific serine ADP-ribosylated protein or peptide.
Dependencies are inferred from the claim text.