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US 12,329,857 B2Granted
Systems and methods for manufacturing lipid nanoparticles and liposomes
AssigneeAcuitas Therapeutics, Inc.
InventorsChristopher J. Barbosa, Kody Moleschi, David Cretney
Life of this patent
Estimating the term…
Abstract
Systems including specific arrangements of pumps, valves, and conduits, such as for mixing precursors to lipid nanoparticles to form the lipid nanoparticles, are provided. Methods of using such systems to manufacture the lipid nanoparticles are also provided.
Claims24
A system, comprising:
- a first reservoir, the first reservoir containing an organic lipid stock solution;
- a first pump having a first pump inlet and a first pump outlet, the first pump inlet fluidically coupled to the first reservoir;
- a second reservoir, the second reservoir containing an aqueous nucleic acid stock solution;
- a second pump having a second pump inlet and a second pump outlet, the second pump inlet fluidically coupled to the second reservoir;
- a valve having a first valve inlet port fluidically coupled to the first pump outlet, a second valve inlet port fluidically coupled to the second pump outlet, a first valve outlet port, a second valve outlet port, a third valve outlet port, and a fourth valve outlet port;
- and
- a mixing assembly having a first mixing assembly inlet fluidically coupled to the first valve outlet port, a second mixing assembly inlet fluidically coupled to the second valve outlet port, and a mixing assembly outlet, wherein the first mixing assembly inlet is fluidically coupled to the mixing assembly outlet and the second mixing assembly inlet is fluidically coupled to the mixing assembly outlet;
- wherein the valve has a first operating position in which the first valve inlet port is fluidically coupled to the first valve outlet port and the second valve inlet port is fluidically coupled to the second valve outlet port and a second operating position in which the first valve inlet port is not fluidically coupled to the first valve outlet port and the second valve inlet port is not fluidically coupled to the second valve outlet port,
- wherein the third valve outlet port is fluidically coupled to the first reservoir in both of the first and second operating positions, and
- wherein the fourth valve outlet port is fluidically coupled to the second fluid reservoir in both of the first and second operating positions.
7 more elements
A method of manufacturing lipid nanoparticles, comprising:
- providing an organic lipid stock solution within a first reservoir, the first reservoir fluidically coupled to a first valve outlet port of a valve in both a first operating position of the valve and a second operating position of the valve;
- providing an aqueous nucleic acid stock solution within a second reservoir, the second reservoir fluidically coupled to a second valve outlet port of the valve in both the first operating position of the valve and the second operating position of the valve;
- pumping the organic lipid stock solution from the first reservoir to a first valve inlet port of the valve;
- pumping the aqueous nucleic acid stock solution from the second reservoir to a second valve inlet port of the valve;
- flowing the organic lipid stock solution and the aqueous nucleic acid stock through the valve and operating the valve in the second operating position in which the first valve inlet port is not fluidically connected to a first mixing assembly inlet of a mixing assembly and the second valve inlet port is not fluidically connected to a second mixing assembly inlet of the mixing assembly in the second operating position;
- and
- switching the valve to the first operating position and flowing the organic lipid stock solution through the valve to the first mixing assembly inlet of a mixing assembly and flowing the aqueous nucleic acid stock solution through the valve to the second mixing assembly inlet of the mixing assembly thereby mixing the organic lipid stock solution and the aqueous nucleic acid stock solution within the mixing assembly to create a first mixture and flowing the first mixture out of the mixing assembly through a mixing assembly outlet.
4 more elements
Dependencies are inferred from the claim text.