US 12,329,789 B2 · Transformed, Salmo salar interferon gamma (IFNg)-producing Lactococcus lactis bacterium, food and composition comprising same, for immunostimulation in aquaculture species

Transformed, Salmo salar interferon gamma (IFNg)-producing Lactococcus lactis bacterium, food and composition comprising same, for immunostimulation in aquaculture species

AssigneesUniversidad de Santiago de Chile, Consorcio Tecnologico De Sanidad Acuicola S.A.

InventorsMario Cesar Gerardo Tello Reyes, Alvaro Eugenio Santibańez Vargas, Mick Philippe Parra Mardonez, Diego Enrique Paine Cabrera, Claudia Andrea Zapata Rojas, Andrea Del Pilar Garces Fernandez

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Abstract

The present invention falls within the technical field of aquaculture, and specifically, the invention relates to a specific solution for preventing and treating bacterial diseases using a Lactococcus lactis lactic acid bacterium transformed to produce an interferon type II (IFN II) immunostimulating cytokine, particularly interferon gamma (IFNg or IFNγ). Said transformed bacterium has been deposited in the Chilean Microbial Genetic Resources Collection at INIA with accession number RGM 2416 dated 22 Oct. 2017. The invention is also related to an immunostimulating food for aquaculture species comprising the lactic acid bacterium transformed with IFN type II from salmonidae. Moreover, the invention relates to the method for preparing said probiotic food, the method for preparing said lactic acid bacterium expressing IFNg, in other words which produces a cytokine that stimulates the antibacterial immune response.

Claims13

Dependent claims

A transformed Lactococcus lactis bacteria having the accession number RGM 2416, comprising SEQ ID NO:

1, wherein SEQ ID NO:
1 is a DNA construct P1-USP45-IFNy-GGG-6xHis, wherein the DNA construct is within a vector pNZ8149, wherein the vector has at least one restriction site, wherein the at least one restriction site comprises a first restriction site for restriction enzyme Xbal and a second restriction site for restriction enzyme Ncol;
P1 is a L. lactis constitutive expression promoter;
Usp45 is a secretion signal;
IFNy is a codon-optimized Salmo salar interferon gamma mature mRNA coding sequence;
GGG is a sequence coding for three glycines;
and 6xHis is a sequence coding for 6 terminal histidines, wherein the transformed L. lactis bacteria is capable of producing Salmo salar interferon gamma.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/764,356Filed 20 Apr 2018

Priority

CL 2897-201714 Nov 2017

Published

US 2022/0347235 A13 Nov 2022

Granted

17 Jun 2025

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Classification24 codes

A61K 35/744
Probiotics: lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
A23K 10/18
Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions: of live microorganisms
A23K 50/80
Feeding-stuffs specially adapted for particular animals: for aquatic animals, e.g. fish, crustaceans or molluscs
A61K 9/0053
Galenical forms characterised by the site of application: mouth and digestive tract, i.e. intraoral and peroral administration