# US 12,329,180 B2 · Soluble rapeseed protein isolate

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- Number: US 12,329,180 B2
- Document ID: US12329180B2
- Status: Granted
- Kind: B2
- Application number: 18/068,798
- Filed: 2022-12-20
- Published: 2023-04-20
- Granted: 2025-06-17
- Earliest claimed: 2016-07-07
- Assignees: Dsm IP Assets B.V.
- Applicants: Dsm IP Assets B.V.
- Inventors: Johannes Hendrikus Maria Willemsen; Johannes Hendrikus Antonius Jeroen Vermunt; Nienke Nina Hylkema; Gerardus Johannes Franciscus Smolders
- Web page: https://patentgrep.com/patent/US12329180B2

## CPC

- A23J 1/14: from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23G 1/44: containing peptides or proteins (containing dairy products A23G1/46)
- A23J 3/14: Vegetable proteins
- A23K 20/147: Polymeric derivatives, e.g. peptides or proteins
- A23K 20/158: Fatty acids; Fats; Products containing oils or fats
- A23K 50/40: for carnivorous animals, e.g. cats or dogs
- A23L 11/30: Removing undesirable substances, e.g. bitter substances
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23G: COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention discloses a native rapeseed protein isolate comprising 40 to 65% cruciferins and 35 to 60% napins and having a solubility of at least 88% when measured over a pH range from 3 to 10 at a temperature of 23±2° C.; wherein the native rapeseed protein isolate comprises from 5% to 65% on dry matter of 12S rapeseed protein where the presence of 12S is verified by Blue Native PAGE.

## Claims

### Claim 1

A native rapeseed protein isolate comprising 40 wt % to 65 wt % cruciferins and 35 wt % to 60 wt % napins and having a solubility of at least 88% when measured over a pH range from 3 to 10 at a temperature of 23±2° C.; wherein the native rapeseed protein isolate comprises from 25 wt % to 65 wt % on dry matter of 12S rapeseed protein, wherein the presence of 12S is verified by Blue Native PAGE, and wherein the native rapeseed protein isolate comprises less than 20 wt % on dry matter of 7S rapeseed protein.

### Claim 2 (depends on 1)

The native rapeseed protein isolate of claim 1, wherein the native rapeseed protein isolate has a conductivity in a 2 wt. % aqueous solution of less than 9,000 μS/cm over a pH range of 2 to 12.

### Claim 3 (depends on 1)

The native rapeseed protein isolate of claim 1, wherein the native rapeseed protein isolate comprises at least 35 wt % on dry matter of 12S rapeseed protein.

### Claim 4 (depends on 1)

The native rapeseed protein isolate of claim 1, wherein the native rapeseed isolate has a cruciferins/napins (C/N) weight ratio in a range of from 0.9 to 1.3.

### Claim 5 (depends on 1)

The native rapeseed protein isolate of claim 1, wherein the native rapeseed protein isolate has a phytate level less than 0.4 wt. %.

### Claim 6 (depends on 1)

The native rapeseed protein isolate of claim 1, wherein the native rapeseed protein isolate has a solubility of at least 94% when measured over a pH range from 3 to 10 at a temperature of 23±2° C.

### Claim 7 (depends on 1)

A food product or a pet food product comprising the native rapeseed protein isolate of claim 1.

### Claim 8 (depends on 7)

The pet food product of claim 7, wherein the food product comprises from 5 wt % to 35 wt % of native rapeseed protein isolate.