Back to results
US 12,326,451 B2Granted
Severe acute respiratory syndrome (SARS)—associated coronavirus diagnostics
AssigneeInstitut Pasteur
InventorsSylvie Van Der Werf, Nicolas Escriou, Caroline Demeret, Stéphane Petres, Pierre Lafaye, Jacques Bellalou
Life of this patent
Estimating the term…
Abstract
The invention relates to the diagnosis of a SARS-associated coronavirus, such as a SARS-CoV-2 infection and SARS-CoV-1 infection, using the N_SARS-CoV-1 and N_SARS-CoV-2 proteins and antibodies binding to these proteins. The invention reagents, methods and kits for the detection of a SARS-associated coronavirus.
Claims18
A kit for detection of a severe acute respiratory syndrome (SARS)-associated coronavirus infection in a biological sample comprising:
- a severe acute respiratory syndrome-associated coronavirus 2 nucleocapsid protein (SARS-CoV-2 N protein) fused to a N-terminally located poly-histidine tag containing 6 histidines, made by a process comprising expressing and purifying said SARS-CoV-2 N protein fused to a N-terminally located poly-histidine tag containing 6 histidines from Escherichia coli (E. coli) strain BL21 (DE3) pDIA17 transformed with recombinant plasmid pETM11/N-nCov E. coli 3-(His)6-Nter or pETM11/N-nCov E. coli 4-(His)6-Nter deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, 25, Rue du Docteur Roux, 75724 Paris, France, on May 11, 2020, under the deposit numbers CNCM 1-5510 and CNCM 1-5511, respectively;
- and
- a severe acute respiratory syndrome-associated coronavirus 2 spike protein (SARS-CoV-2 S protein).
Dependencies are inferred from the claim text.