Microbial approach for the production of long chain compounds
AssigneesUT-Battelle, LLC, University of Georgia Research Foundation, Inc., University of Tennessee Research Foundation
InventorsJanet Westpheling, Adam M. Guss, Lauren A. Riley
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Abstract
This disclosure describes recombinant Megasphaera microbes designed to include increased consumption of acetate, increased carbon flux to butyryl-CoA and/or hexanoyl-CoA, increased production of butyrate and/or hexanoate, or a combination thereof, than a comparable control. This disclosure also describes methods that generally include growing such recombinant microbes under conditions effective for the recombinant microbes to consume greater amounts of acetate, produce increased amounts of butyryl-CoA and/or hexanoyl-CoA, produce increased amounts of butyrate and/or hexanoate, or a combination thereof.
Claims24
A recombinant Megasphaera microbe genetically modified to (i) consume a greater amount of acetate than a comparable control microbe, or (ii) produce a greater amount of butyrate, hexanoate, or a combination thereof, than a comparable control microbe, or (iii) increase carbon flux to butyryl-CoA and/or hexanoyl-CoA than a comparable control microbe, or a combination thereof, wherein the recombinant Megasphaera microbe comprises a mutation of a CoA-transferase coding region, a mutation of a glyoxalase coding region, or a mutation of a lyase coding region, and the comparable control microbe is a microbe genetically identical to the recombinant Megasphaera microbe except for the mutation of the CoA-transferase coding region, the glyoxalase coding region, or the lyase coding region.
A recombinant Megasphaera microbe comprising a mutation of a pyrF coding region to result in undetectable expression of the pyrF coding region (SEQ ID NO:
- 29).
Dependencies are inferred from the claim text.