Chlamydomonas mutant and use thereof
AssigneeIndustry-University Cooperation Foundation Hanyang University
InventorsEon Seon Jin, Kwang Ryul Baek, In Hwa Song
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Abstract
The present invention relates to a novel Chlamydomonas strain with an improved oil generation function, the strain of the present invention having useful mycological characteristics as a strain that provides a useful substance, such as a vegetable oil, in a microalga, as the strain has a fast cell growth speed and an excellent lipid generation function compared to conventional strains. In particular, the present invention can provide a vegetable oil with improved stability and a longer preservation period by containing, in a cell, a large amount of antioxidant pigments such as lutein and zeaxanthin, and can, thereby, be usefully used in industries such as food, medicine, cosmetics, etc., which utilize a vegetable oil.
Claims14
A Chlamydomonas reinhardtii mutant strain, comprising:
- a ZEP gene mutant in which adenine (A), a base sequence of SEQ ID NO:
- 3 or a base sequence of SEQ ID NO:
- 5 is inserted between an 816th base and an 817th base in a Chlamydomonas reinhardtii ZEP gene sequence of SEQ ID NO:
- 1;
- and
- an AGP gene mutant in which adenine (A) is inserted between a 116th base and a 117th base in a Chlamydomonas reinhardtii AGP gene sequence of SEQ ID NO:
- 2, otherwise, a 115th base is deleted while the 116th base is substituted by thymine (T).
A method for preparation of Chlamydomonas reinhardtii mutant strain having a ZEP gene mutation, an AGP gene mutation, and an improved oil production ability, said method comprising:
- mutating a ZEP gene by targeting 800th to 820th bases in the ZEP gene sequence of Chlamydomonas reinhardtii, wherein a wild-type ZEP gene sequence is a sequence of SEQ ID NO:
- 1;
- and
- mutating an AGP gene by targeting 100th to 120th bases in the AGP gene sequence of Chlamydomonas reinhardtii, wherein a wild-type AGP gene sequence is a sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.