Enzyme-linked immunosorbent assay (ELISA) method and monoclonal antibody for giant panda relaxin-3 (RLN3)

Abstract

An enzyme-linked immunosorbent assay (ELISA) method and a monoclonal antibody for giant panda relaxin-3 (RLN3) are provided. The method includes: conducting ELISA with an anti-giant panda RLN3 monoclonal antibody as a coating antibody and an anti-RLN3 polyclonal antibody as a labeling antibody, where the anti-giant panda RLN3 monoclonal antibody is secreted by a hybridoma cell line RLN-3; and the hybridoma cell line RLN-3 was deposited in the China Center for Type Culture Collection (CCTCC) of Wuhan University, Wuhan, China on Jan. 7, 2021, with an accession number of CCTCC NO: C202129. In addition, an ELISA method for detecting RLN3 in giant panda urine is developed, in which a prepared anti-giant panda RLN3 monoclonal antibody is used as a coating antibody and an anti-giant panda RLN3 polyclonal antibody is used as a labeling antibody. The method can monitor a change in giant panda RLN3 timely by determining giant panda RLN3.

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