Enzymatically synthesized omega-3 structured phospholipids
AssigneeC.I. Naturmega S.A.
InventorsWilson Martinez Gutierrez, Alvaro Jose Garcia Padilla, Alfredo de Jesús Puche Simanca, Tatiana Lucia Yepes Bustillo
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Abstract
The invention provides a process of incorporation of omega-3 fatty acids such as EPA/DHA into polar lipid molecules present in lecithin, which consists of: (a) an enzymatic exchange reaction between the fatty acids present in the polar lipids of lecithin and the omega-3 fatty acids present in concentrated fish oil, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and (b) a stage of concentration of the polar lipid content of the oil obtained in stage a, by supercritical fractionation or molecular distillation.
Claims14
A process for the incorporation of omega-3 and omega-6 fatty acids into polar lipid molecules present in phospholipids and glycolipids, which process comprises the steps of:
- (a) conducting an enzymatic exchange reaction between the fatty acids present in the polar lipids of the phospholipids and glycolipds and the omega-3 and omega-6 fatty acids, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and omega-6 fatty acids, said enzymatic exchange reaction being conducted in the presence of a lipase or phospholipase enzyme selected from the group consisting of a chimera produced by the fusion of the genes of the lipase from Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum (Lecitase Ultra), Phospholipase C (Purifine), and Candida antarctica lipase B (CALB);
- (b) a stage of concentration of the polar lipid and omega-3 and omega-6 fatty acids content of the oil obtained in step (a) by supercritical fractionation or molecular distillation;
- (c) a new feeding stage of concentrated omega-3 oil or omega-6 to increase the incorporation of omega-3 and omega-6 in polar lipids and achieve a higher omega-3 and omega-6 content in the product;
- and (d) an ultrafiltration stage under a nitrogen atmosphere to give the desired consistency and appearance to the final oil.
A process for the incorporation of EPA/DHA into polar lipid molecules present in lecithin, which process comprises the following steps:
- (a) an enzymatic exchange reaction between the fatty acids present in the polar lipids of lecithin and the omega-3 fatty acids present in concentrated fish oil or algae oil, to obtain an oil with a high content of polar lipids and omega-3 fatty acids, said enzymatic exchange reaction being conducted in the presence of a lipase or phospholipase enzyme selected from the group consisting of a chimera produced by the fusion of the genes of the lipase from Thermomyces lamiginosus and the phospholipase A1 from Fusarium oxysporum (Lecitase Ultra), Phospholipase C (Purifine), and Candida antarctica lipase B (CALB);
- (b) a stage of concentration of the polar lipid and omega-3 fatty acids content of the oil obtained in stage a, by supercritical fractionation or molecular distillation;
- (c) a new feeding stage of concentrated Omega-3 oil (fish or algae) to increase the incorporation of Omega-3 in polar lipids and achieve a higher omega-3 content in the product;
- and (d) an ultrafiltration stage under a nitrogen atmosphere to give the desired consistency and appearance to the final oil.
Dependencies are inferred from the claim text.