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US 12,319,947 B2Granted
Method for preparing (S)-nicotine by reduction
AssigneePorton Pharma Solutions Ltd.
InventorsWenqing Lin, Hongjie Zheng, Xiaobo Liu, Zecong Chen, Lingyu Li, Qingjun Zhou, Songhe Wang, Yongtang Yue, Jicheng Hu, Yue Zhang, Shanshan Miao
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Abstract
A method for preparing (S)-nicotine by reduction includes conducting a reduction process on an alkene compound as shown in Formula I and/or an iminium cation compound as shown in Formula II, thereby producing (S)-nicotine. The method is simple, safe, reliable, and yields both high purity and high quantities of (S)-nicotine production.
Claims10
A method for preparing (S)-nicotine by reduction, the method comprising:
- conducting a reduction process on an alkene compound shown in Formula I and/or an iminium cation compound shown in Formula II
- thereby producing (S)-nicotine;
- wherein
- the reduction process is performed using a biocatalytic method;
- the biocatalytic method comprises:
- in a coenzyme cycling system, catalytically reducing the alkene compound shown in Formula I and/or the iminium cation compound shown in Formula II by an imine reductase, thereby producing (S)-nicotine;
- the coenzyme cycling system further comprises a coenzyme, glucose, and glucose dehydrogenase;
- the imine reductase comprises an amino acid sequence selected from SEQ ID NOs:
- 2-4, 6, and 12-22;
- the reduction process is carried out within a temperature range of 15 to 45° C.;
- and
- the reduction process is carried out within a pH range of 6.0 to 8.0.
9 more elements
Dependencies are inferred from the claim text.