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US 12,319,946 B2Granted
Methods for producing modified bacteria for production of nitroaromatics
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYousong Ding, Ran Zuo
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Abstract
The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.
Claims16
A method of producing a recombinant bacterial cell comprising
- (i) transforming a bacterial cell with an isolated nucleic acid comprising the sequence set forth in any one of SEQ ID NOs:
- 8-13;
- and,
- (ii) culturing the bacterial cell in a bacterial culture media.
1 more element
Dependencies are inferred from the claim text.