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US 12,319,938 B2Granted
Enhanced virus-like particles and methods of use thereof for delivery to cells
AssigneesThe General Hospital Corporation, President and Fellows of Harvard College
InventorsJ. Keith Joung, Peter Cabeceiras
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Abstract
Enhanced virus-like particles (eVLPs), comprising a membrane comprising a phospholipid bilayer with one or more virally-derived glycoproteins on the external side; and a cargo disposed in the core of the eVLP on the inside of the membrane, wherein the eVLP does not comprise an exogenous gag/pol protein, and methods of use thereof for delivery of the cargo to cells.
Claims37
A particle for delivering a CRISPR-based genome editing protein to a nucleus of a target cell, the particle comprising:
- (a) a membrane comprising a phospholipid bilayer with a glycoprotein on an external side;
- and
- (b) a fusion protein comprising the CRISPR-based genome editing protein fused via a linker with a non-viral plasma membrane recruitment domain comprising a pleckstrin homology (PH) domain disposed in a core of the particle, and
- wherein the fusion protein does not comprise a Gag protein.
1 more element
Dependencies are inferred from the claim text.