Apparatus for polymerase chain reaction of nucleic acid

An apparatus for thermal cycling for a polymerase chain reaction (PCR) of nucleic acid, wherein the apparatus employs a reactor holder for holding at least one reactor to accommodate a reaction material containing the nucleic acid, and the at least one reactor is reactor in a form selected from the group consisting of capillaries, tubes, wellplates, chips, and cartridges, and the apparatus comprises:

• two baths comprising a first bath and a second bath, wherein in use, bath media comprising a bath medium in the first bath and a bath medium in the second bath are respectively maintainable at two different temperatures;
• a powder-removing device such that in operation, before an imaging, the powder-removing device mechanically removes particles of a powder adhering to the at least one reactor, wherein the powder is at least one of the bath media in use;
• a transfer means for allowing the at least one reactor to be in the two baths in a plurality of thermal cycles to alternately attain:
• a predetermined high target temperature THT, and
• a predetermined low target temperature TLT;
• a florescent imaging means for imaging the reaction material during or after the thermal cycling.