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US 12,318,506 B2Granted
Method for manufacturing ring-shaped bone grafting substitute
AssigneePurgo Biologics Inc.
InventorHo Chang Kang
Life of this patent
Estimating the term…
Abstract
Provided is a method for manufacturing a ring-shaped bone grafting substitute. The method for manufacturing a ring-shaped bone grafting substitute includes a biodegradable polymer providing step of providing a biodegradable polymer, a molding material providing step of providing a molding material in which the biodegradable polymer and a bone material are mixed; a molding material injection step of injecting the molding material into a ring-shaped mold such that a hole is formed in the center thereof; and ring-shaped bone grafting substitute molding step of freeze-drying the molding material having been injected into the ring-shaped mold at a temperature lower than a predetermined reference temperature, thereby molding the resultant into a ring-shaped bone grafting substitute.
Claims15
A method for manufacturing a ring-shaped bone grafting substitute, the method comprising:
- a biodegradable polymer providing step of providing a biodegradable polymer;
- a molding material providing step of providing a molding material by mixing the biodegradable polymer with a bone material;
- a molding material injection step of injecting the molding material into a ring-shaped mold having a ring shape to form a hole at a center;
- and
- a ring-shaped bone grafting substitute molding step of molding a ring-shaped bone grafting substitute having a ring shape by freeze-drying the molding material injected into the ring-shaped mold at a temperature lower than a predetermined reference temperature,
- wherein, in the molding material providing step, the bone material and the biodegradable polymer are mixed such that a weight ratio of bone material/biodegradable polymer is 60/40 to 95/5,
- wherein the biodegradable polymer comprises collagen, the collagen comprises collagen from porcine skin, and a collagen providing step of providing the collagen comprises:
- a porcine skin preprocessing step of preprocessing the porcine skin to obtain collagen from the porcine skin;
- a homogenization step of dissolving the preprocessed porcine skin in a solvent to produce a homogenized collagen solution;
- and
- a collagen filtering step of filtering collagen from the homogenized collagen solution,
- wherein the collagen comprises crosslinked collagen that is crosslinked to have a crosslink structure,
- wherein the method further comprises, after the collagen filtering step:
- a fibrillation buffer mixing step of providing a mixed solution obtained by mixing the collagen obtained from the collagen filtering step with a fibrillation buffer having sodium chloride 20-30 part by weight, sodium hydroxide 1-3 part by weight, and di-sodium hydrogen phosphate dihydrate 3-5 part by weight with respect to water 100 part by weight;
- a gel state change step of providing a mixture in a gel state by mixing the mixed solution with gamma polyglutamic acid (y-PGA) and putting a resultant therefrom in a well plate to be changed to a gel state in an incubator;
- a crosslink reaction processing step of forming crosslinked collagen by mixing the gel state mixture with a crosslinking solution to induce a crosslink reaction;
- a washing step of washing the crosslinking solution mixed in the crosslink reaction processing step;
- an after-crosslink-processing homogenization step of dissolving the crosslinked collagen in a solvent to produce a homogenized crosslinked collagen solution;
- and
- a crosslinked collagen filtering step of filtering crosslinked collagen from the homogenized crosslinked collagen solution.
17 more elements
A method for manufacturing a ring-shaped bone grafting substitute, the method comprising:
- a biodegradable polymer providing step of providing a biodegradable polymer;
- a molding material providing step of providing a molding material by mixing the biodegradable polymer with a bone material;
- a molding material injection step of injecting the molding material into a ring-shaped mold having a ring shape to form a hole at a center;
- and
- a ring-shaped bone grafting substitute molding step of molding a ring-shaped bone grafting substitute having a ring shape by freeze-drying the molding material injected into the ring-shaped mold at a temperature lower than a predetermined reference temperature, wherein, in the molding material providing step, the bone material and the biodegradable polymer are mixed such that a weight ratio of bone material/biodegradable polymer is 60/40 to 95/5,
- wherein the biodegradable polymer comprises collagen, the collagen comprises collagen from porcine ligament, and the collagen providing step of providing the collagen comprises:
- a pig ligament preprocessing step of preprocessing the pig ligament to obtain collagen from the pig ligament;
- a collagen dough molding step of molding a preprocessed pig ligament into a collagen dough;
- a homogenization step of dissolving the collagen dough in a solvent to produce a homogenized collagen solution;
- and
- a collagen filtering step of filtering collagen from the homogenized collagen solution, and
- wherein the collagen comprises crosslinked collagen that is crosslinked to have a crosslink structure, the method further comprising, after the collagen filtering step:
- a fibrillation buffer mixing step of providing a mixed solution obtained by mixing the collagen obtained from the collagen filtering step with a fibrillation buffer having sodium chloride 20-30 part by weight, sodium hydroxide 1-3 part by weight, and di-sodium hydrogen phosphate dihydrate 3-5 part by weight with respect to water 100 part by weight;
- a gel state change step of providing a mixture in a gel state by mixing the mixed solution with gamma polyglutamic acid (γ-PGA) and putting a resultant therefrom in a well plate to be changed to a gel state in an incubator;
- a crosslink reaction processing step of forming crosslinked collagen by mixing the gel state mixture with a crosslinking solution to induce a crosslink reaction;
- a washing step of washing the crosslinking solution mixed in the crosslink reaction processing step;
- an after-crosslink-processing homogenization step of dissolving the crosslinked collagen in a solvent to produce a homogenized crosslinked collagen solution;
- and
- a crosslinked collagen filtering step of filtering crosslinked collagen from the homogenized crosslinked collagen solution.
16 more elements
Dependencies are inferred from the claim text.