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US 12,318,442 B2Granted
Preparation of influenza virus vaccine antigens
AssigneeSeqirus UK Limited
InventorsChristoph Haussmann, Frank Hauschild, Bjorn Jobst
Life of this patent
Estimating the term…
Abstract
A number of improvements for preparing vaccine antigens from disintegrated influenza viruses are disclosed. A splitting step can be followed by detergent exchange. Splitting can take place in the presence of a buffer with a higher ionic strength and/or in the presence of phosphate buffer.
Claims19
A method for disrupting influenza virions, the method comprising steps of:
- (i) obtaining a composition comprising influenza virions prepared from a cell culture,
- (ii) capturing the influenza virions in the composition via filtration;
- (iii) adding a first detergent to the filtered composition without splitting the virions;
- (iv) inactivating the influenza virions to obtain inactivated virions;
- and
- (v) splitting the inactivated virions in the presence of a second detergent different from the first detergent;
- wherein disrupting the influenza virions is a process with a phosphate buffer having a pH between 6.5 and 8.5 and an ionic strength of from 100 mM to 800 mM, the higher ionic strength being achieved by monovalent ions, divalent ions, or trivalent ions.
4 more elements
Dependencies are inferred from the claim text.