Methods and system for image guided cell ablation

An in vivo method of treating a subject comprising:

• (a) administering a composition comprising a molecular imaging probe to the subject, wherein the molecular imaging probe labels diseased cells;
• (b) permitting the molecular imaging probe to clear preferentially from healthy tissue leaving the diseased cells labeled;
• (c) performing initial resection surgery to remove diseased cells from the subject thereby creating a resection bed;
• (d) directing light towards the resection bed to excite the molecular imaging probe;
• (e) obtaining an in situ fluorescence image of the resection bed using an imaging system;
• and
• (f) detecting the labeled diseased cells, if present in the resection bed, using the in situ fluorescence image.