Compositions and methods for cotton cell culture

Abstract

A method of producing cotton fibers from cotton callus cells in vitro is provided. The method includes the following steps in order: (1) culturing cotton callus cells in a suspension culture medium; (2) feeding a first bioreactor with the callus cells, a multiplication medium, and air compression; (3) culturing the callus cells in the first bioreactor for 5 to 12 days to accomplish at least two rounds of duplication of the callus cells; (4) serially repeating steps (2) and (3) one or more times, thereby obtaining serially duplicated cells; (5) seeding one or more second bioreactors with the serially duplicated cells, an elongation medium, and air compression; (6) culturing the serially duplicated cells in the one or more second bioreactors, thereby obtaining elongated cells; and (7) culturing the elongated cells in a maturation medium, thereby obtaining the cotton fibers.

Dependencies are inferred from the claim text.