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US 12,313,623 B2Granted
Blood separation and analysis device and methods
Assignee1866402 Ontario Limited
InventorJeffrey Sutton
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Abstract
There is provided a device and method for separation of blood, including sedimentation of plasma using PVA. The device comprises an inner container enclosed in an outer container, wherein upon alignment of respective openings, allows sample to exit from the inner container into a reaction structure. The reaction structure comprises one or more layers, each with one or more portions each containing concentrations of one or more chemicals.
Claims9
A method for determining the amount of an analyte of interest in an unknown quantity of sample comprising:
- providing three reaction channels each containing a reagent that reacts with the analyte to produce either a first detectable signal proportional to the amount of the analyte in the reaction channel or a reaction product that can be further reacted to produce the first detectable signal proportional to the amount of the analyte in the reaction channel,
- the first reaction channel containing a known first quantity of the analyte of interest n,
- the second reaction channel containing a known second quantity of the analyte of interest m, wherein m is different than n,
- and the third reaction channel containing a known third quantity of the analyte of interest o, wherein o≠m or n,
- depositing a volume of the sample in each of the reaction channels;
- measuring the first detectable signal produced in each reaction channel;
- and
- determining the amount of analyte in the unknown sample based on the method of standard addition.
5 more elements
Dependencies are inferred from the claim text.