LAMP assay device

A loop-mediated isothermal amplification (LAMP) assay device comprising:

• a heating chamber wherein the heating chamber comprises:
• at least two internal heating elements;
• a circuit of light-emitting elements positioned on one of a front wall or a rear wall of the heating chamber;
• and
• a circuit of light sensors positioned opposite the circuit of light-emitting elements on one of the front wall or the rear wall of the heating chamber;
• a removeable support rail wherein the heating chamber is configured to receive the removeable support rail configured to hold one or more samples when the removeable support rail is inserted into the heating chamber through a sample insertion opening;
• and
• a real-time control system wherein the real-time control system is configured to control a temperature of the heating chamber during a stabilization cycle while the removeable support rail is outside the heating chamber by controlling current flow through the at least two internal heating elements fixed inside the heating chamber,
• wherein after the stabilization cycle, the real-time control system is configured to provide thermal control exclusively based on thermal inertia of the at least two internal heating elements of the heating chamber during a reaction mode based on a speed with which an environment inside the heating chamber gains or loses heat to an environment outside the heating chamber while the removeable support rail is inserted into the heating chamber,
• wherein for each sample of the one or more samples that the removeable support rail is configured to hold, one of the light-emitting elements is configured to be positioned opposite one of the light sensors,
• wherein one of the internal heating elements is positioned in a lower portion of the heating chamber, and another one of the internal heating elements is positioned in an upper portion of the heating chamber,
• wherein the at least two internal heating elements are of a resistive type and are fixed inside the heating chamber, and
• wherein the internal heating element positioned in the upper portion comprises a heating circuit with a first resistance value and the internal heating element positioned in the lower portion comprises a heating circuit with a second resistance value, the first resistance value being less than the second resistance value.

A loop-mediated isothermal amplification (LAMP) assay device comprising:

• a heating chamber wherein the heating chamber comprises:
• at least two internal heating elements;
• a circuit of light-emitting elements positioned on one of a front wall or a rear wall of the heating chamber;
• and
• a circuit of light sensors positioned opposite the circuit of light-emitting elements on one of the front wall or the rear wall of the heating chamber;
• a removeable support rail wherein the heating chamber is configured to receive the removeable support rail configured to hold one or more samples when the removeable support rail is inserted into the heating chamber through a sample insertion opening;
• and
• a real-time control system wherein the real-time control system is configured to control a temperature of the heating chamber during a stabilization cycle while the removeable support rail is outside the heating chamber by controlling current flow through the at least two internal heating elements fixed inside the heating chamber,
• wherein, after the stabilization cycle, the real-time control system is configured to provide thermal control exclusively based on thermal inertia of the at least two internal heating elements of the heating chamber during a reaction mode based on a speed with which an environment inside the heating chamber gains or loses heat to an environment outside the heating chamber while the removeable support rail is inserted into the heating chamber,
• wherein for each sample of the one or more samples that the removeable support rail is configured to hold, one of the light-emitting elements is configured to be positioned opposite one of the light sensors,
• wherein one of the internal heating elements is positioned in a lower portion of the heating chamber, and another one of the internal heating elements is positioned in an upper portion of the heating chamber,
• wherein the at least two internal heating elements are of a resistive type and are fixed inside the heating chamber, and
• wherein the internal heating element positioned in the upper portion comprises a heating circuit with a first resistance value and the internal heating element positioned in the lower portion comprises a heating circuit with a second resistance value, the first resistance value being less than the second resistance value,
• wherein the heating chamber comprises a temperature sensor positioned in an intermediate region of the heating chamber, and
• wherein the real-time control system is configured to communicate with the at least two internal heating elements, the circuit of light-emitting elements, the circuit of light sensors, and the temperature sensor, and is configured to control the at least two internal heating element during a stabilization cycle while the removeable support rail is outside the heating chamber and during the reaction mode while the removeable support rail is inside the heating chamber.