Method for detecting chromosomal abnormalities

A method for detecting at least two chromosome abnormalities that are different from one another by in situ hybridization to detect chromosome regions and/or DNA regions in a biological sample, the method comprising:

• providing a mixture of at least four locus-specific hybridization probes comprising:
• a) a first probe pair configured to hybridize with a nucleic acid flanking a first chromosome region and/or DNA region distally and proximally, marked with first detection labels that are different from one another, so that a fusion signal is generated in a signal pattern when the first probe pair flanks the first chromosome region and/or DNA region, and
• b) a second probe pair configured to hybridize with a nucleic acid flanking a second chromosome region and/or DNA region distally and proximally, marked with first detection labels that are different from one another, so that a fusion signal is generated in a signal pattern when the second probe pair flanks the second chromosome region and/or DNA region,
• wherein for generation of at least one mixed signal, at least one of the locus-specific hybridization probes is marked with at least one further detection label, different from all of the first detection labels,
• applying the mixture of the at least four locus-specific hybridization probes to the biological sample;
• hybridizing the mixture with the biological sample, and
• detecting one or more of a fusion signal, mixed signal, and/or single signal from said detection labels so that a signal pattern is generated to thereby detect said chromosome abnormalities, chromosome regions, and/or DNA regions in said biological sample,
• wherein the hybridization probes are labeled directly with the detection labels, and the hybridizing step is carried out as interphase/in situ hybridization.