High-throughput multiplexed sensitivity and resistance assay

Abstract

In the clinic, most patients' cancer tissues have a heterogeneous histological appearance, often as a result of newly emerging and resistant cancer cell populations. Thus, viability tests to detect the sensitivity of a cancer tissue might not accurately predict a patient's tumor response if smaller but resistant cancer cells proliferate and replace the cells that are sensitive to the current treatment. Provided are methods to multiplex the detection of cell viability and proliferation in a single sample. A cell or tissue sample is treated, incubated with a glycosylamine, and subsequently washed. The sample is then permeabilized, and a first detectable marker is measured to quantify viability effects while a second detectable marker is measured to quantify proliferative effects. These methods may be used to more reliably predict treatment effects for a cancer patient and to evaluate a biological effect of a therapy on malignant viable cells.

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