Lipid-encapsulated dual-cleaving endonuclease for DNA and gene editing
AssigneeSpecific Biologics Inc.
InventorsDavid R. Edgell, Thomas A. McMurrough, Brent E. Stead, Odisho K. Israel
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Abstract
Provided herein are modified Staphylococcus aureus Cas9 nucleases and methods of using modified Staphylococcus aureus Cas9 nucleases. Also provided herein are chimeric nucleases comprising an I-Tevl nuclease domain, and a Staphylococcus aureus Cas9 and methods of using the chimeric nuclease. Also provided herein are methods to edit genes by administering a chimeric nuclease to a cell or organism without the use of a viral vector.
Claims19
A method to edit genomic DNA of a mammalian cell comprising the step of administering a chimeric nuclease, the chimeric nuclease comprising a modified Enterobacteria Phage T4 (I-TevI) nuclease domain, a linker, an RNA-guided nuclease Staphylococcus aureus Cas9 and a guide RNA to the cell, thereby editing the genomic DNA of the cell, wherein the RNA-guided nuclease Staphylococcus aureus Cas9 comprises the sequence of SEQ ID NO:
- 13 with an aspartic acid to glutamic acid substitution at an amino acid corresponding to position 10 of SEQ ID NO:
- 13.
Dependencies are inferred from the claim text.