Acid phosphatase mutants and methods of using the same

An acid phosphatase mutant, wherein the acid phosphatase mutant is from Pseudomonas aeruginosa (P. aeruginosa),

• wherein the acid phosphatase mutant comprises the amino acid sequence of SEQ ID NO:1 with mutations at any one or more of sites 57, 58, 94, or 135 of SEQ ID NO:
• 1,
• wherein the acid phosphatase mutant possesses a catalytic efficiency that is greater than the catalytic efficiency of a corresponding wild type acid phosphatase from P. aeruginosa, and
• wherein the mutations are in any one or more of (a)-(f):
• (a) the site 58 is mutated into phenylalanine;
• (b) the site 94 is mutated into phenylalanine;
• (c) the site 135 is mutated into arginine;
• (d) the site 94 is mutated into phenylalanine, and the site 135 is mutated into arginine;
• (e) the site 58 is mutated into proline, and the site 135 is mutated into arginine;
• or
• (f) the site 57 is mutated into histidine, the site 58 is mutated into proline, and the site 135 is mutated into arginine.