Vaccinia capping enzyme compositions and methods
AssigneeNew England Biolabs, Inc.
InventorsSaulius Vainauskas, Siu-hong Chan, Christopher H. Taron
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Abstract
The present disclosure relates, according to some embodiments, to compositions, methods, and/or kits for producing vaccinia capping enzyme. For example, active, heterodimers of vaccinia capping enzyme may be produced as fusions comprising D1 and D12 subunits. Vaccinia capping enzyme fusion proteins may further comprise a linker.
Claims14
A vaccinia capping enzyme fusion transcript comprising, in a 5′ to 3′ direction:
- (a) a sequence encoding a D1 subunit, (b) a sequence encoding a linker, and (c) a sequence encoding a D12 subunit, wherein the linker has at least 90% identity to SEQ ID NO:
- 3.
A composition comprising a vaccinia capping enzyme fusion transcript, wherein the vaccinia capping enzyme fusion transcript comprises, in a 5′ to 3′ direction:
- (a) a sequence encoding a D1 subunit;
- (b) a sequence encoding a linker;
- and
- (c) a sequence encoding a D12 subunit, wherein the linker has at least 90% identity to SEQ ID NO:
- 3.
A method for producing an active vaccinia capping enzyme comprising:
- contacting
- (a) a vaccinia capping enzyme fusion transcript comprising, in a 5′ to 3′ orientation,
- (i) a sequence encoding D1, (ii) a sequence encoding a linker having at least 90% identity to SEQ ID NO:
- 3, and
- (iii) a sequence encoding D12;
- with
- (b) an expression system.
Dependencies are inferred from the claim text.