Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
AssigneeCodexis, Inc.
InventorsNicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
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Abstract
The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Claims20
An engineered polypeptide comprising an amino acid sequence with at least 90% sequence identity to SEQ ID NO:6 and two or more residue differences as compared to SEQ ID NO:6 selected from X153Y, X283V, X82P, X141W, X154F, X259I/L/M, X274L/M, and X296N/V;
- wherein the polypeptide has imine reductase activity.
Dependencies are inferred from the claim text.