Retroviral vector
AssigneeOxford Biomedica (UK) Limited
InventorsHannah Stewart, Helen Maunder, Kyriacos Mitrophanous, Daniel Farley
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Abstract
A cell for producing retroviral vectors comprising nucleic acid sequences encoding: i) gag-pol; ii) env; iii) the RNA genome of the retroviral vector; and iv) optionally rev, or a functional substitute thereof, wherein at least two nucleic acid sequences are located at the same genetic locus; and wherein the at least two nucleic acid sequences are in reverse and/or alternating orientations.
Claims12
A bacterial plasmid comprising nucleic acid sequences encoding env and rev in reverse orientations, wherein the nucleic acid sequences encoding env and rev are each operably linked to at least one transcription regulation element selected from the tetracycline repressor (TetR) group of transcription regulators or from the cumate inducible switch system transcription regulators.
A cell comprising a retroviral vector comprising nucleic acid sequences encoding:
- i) gag-pol;
- ii) env;
- iii) rev, and
- iv) optionally, the RNA genome of the retroviral vector;
- wherein the nucleic acid sequences encoding rev and env are located at the same genetic locus;
- wherein the nucleic acid sequences encoding rev and env are in reverse orientations;
- wherein the nucleic acid sequences encoding rev and env are stably integrated into the genome of the cell;
- and wherein the nucleic acid sequences encoding env and rev are each operably linked to at least one transcription regulation element selected from the tetracycline repressor (TetR) group of transcription regulators or from the cumate inducible switch system transcription regulators.
A bacterial plasmid comprising nucleic acid sequences encoding env, rev, and gag-pol, wherein the nucleic acid sequences encoding env and rev are in reverse orientations to one another;
- and wherein the nucleic acid sequences encoding env, rev, and gag-pol are each operably linked to at least one transcription regulation element selected from the tetracycline repressor (TetR) group of transcription regulators or from the cumate inducible switch system transcription regulators.
Dependencies are inferred from the claim text.