Display substrate, preparation method thereof, and display apparatus

A display substrate, comprising:

• a substrate, a drive circuit layer disposed on the substrate and a light emitting structure layer disposed on a side of the drive circuit layer away from the substrate, wherein the drive circuit layer comprises a transistor, and the light emitting structure layer comprises a first electrode, a pixel define layer, an organic light emitting layer and a second electrode, wherein the first electrode is connected with a drain electrode of the transistor, and the organic light emitting layer is located between the first electrode and the second electrode;
• the pixel define layer comprises a plurality of first retaining walls and a plurality of second retaining walls, wherein a height of the first retaining walls is less than a height of the second retaining walls;
• the first retaining walls and the second retaining walls are intersected to define a plurality of pixel opening regions;
• the pixel opening regions comprise a first pixel opening region and a second pixel opening region;
• an area of the first pixel opening region is larger than an area of the second pixel opening region;
• and the first pixel opening region and the second pixel opening region adjacent to each other are separated by a first retaining wall,
• wherein the second retaining wall comprises a first sub-retaining wall and a second sub-retaining wall disposed at intervals, wherein the first sub-retaining wall is provided with a plurality of first bending parts and a plurality of second extending parts which are alternately disposed;
• the first bending parts extend in a first direction, and the second extending parts extend in a second direction.