US 12,306,098 B2 · Methods and related aspects for analyzing exosomes

Abstract

Provided herein are methods of detecting exosomes, including unlabeled exosomes. In some embodiments, the methods include disposing a fluidic sample that comprises a plurality of exosomes in a chamber that is positioned at least partially within a fluidic device in which an inner surface of the chamber comprises a first set of exosome binding moieties that are capable of binding the exosomes. In some embodiments, the methods also include binding a portion of the plurality of exosomes to a portion of the first set of exosome binding moieties to produce surface-bound exosomes, introducing an incident light toward the inner surface of the chamber prior to, concurrent with, and/or after, producing the surface-bound exosomes, and detecting light scattered by the surface-bound exosomes to produce a set of exosome imaging data. Related fluidic devices, systems, and computer readable media are also provided.

Claims20

Dependent claims

A method of detecting an exosome, the method comprising:

disposing a fluidic sample that comprises a plurality of exosomes in a chamber that is positioned at least partially within a fluidic device, wherein at least one inner surface of the chamber comprises at least a first set of exosome binding moieties that are capable of binding the exosomes;
binding at least a portion of the plurality of exosomes to at least a portion of the first set of exosome binding moieties to produce one or more surface-bound exosomes;
introducing an incident light toward the inner surface of the chamber prior to, concurrent with, and/or after, producing the surface-bound exosomes;
and,
detecting light scattered by the surface-bound exosomes to produce a set of exosome imaging data, thereby detecting the exosome.

2 more elements

A system for detecting exosomes, comprising:

a fluidic device receiving area configured to receive a fluidic device that comprises a body structure that defines at least one chamber disposed substantially within the body structure, wherein the chamber comprises an inner surface that is coated with a metallic layer that is configured to create surface plasmon resonance when incident light is introduced toward the inner surface at a suitable incident angle via an outer surface of the chamber, and wherein the metallic layer comprises at least a first set of exosome binding moieties;
a light source configured to introduce an incident light toward the fluidic device receiving area;
a detector configured to collect light scattered by surface-bound exosomes when the fluidic device is received in the fluidic device receiving area and the incident light is introduced from the light source;
and
a controller that comprises, or is capable of accessing, computer readable media comprising non-transitory computer-executable instructions which, when executed by at least one electronic processor, perform at least:
disposing a fluidic sample that comprises a plurality of exosomes in the chamber of the fluidic device such that at least a portion of the plurality of exosomes bind to at least a portion of the first set of exosome binding moieties to produce one or more surface-bound exosomes when the fluidic device is received in the fluidic device receiving area;
introducing the incident light from the light source at the suitable incident angle toward the inner surface of the chamber when the fluidic device is received in the fluidic device receiving area;
and,
detecting light scattered by the surface-bound exosomes over a duration to produce a set of exosome imaging data to thereby detect the exosomes using the detector when the fluidic device is received in the fluidic device receiving area.

6 more elements

Dependencies are inferred from the claim text.

Methods and related aspects for analyzing exosomes

Life of this patent

Methods and related aspects for analyzing exosomes

Life of this patent

Abstract

Claims20