Methods and systems for integrated on-chip single-molecule detection
AssigneeNautilus Subsidiary, Inc.
InventorsPierre Indermuhle, Elliott Sorelle, David Stern, Parag Mallick, Sujal M. Patel
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Abstract
The present disclosure provides methods and systems for performing single-molecule detection using fabricated integrated on-chip devices. Provided herein is a composition, comprising: (a) a substrate; (b) a structured nucleic acid particle coupled to the substrate, wherein a biological entity is coupled to the structured nucleic acid particle; and (c) a fluorescent detection agent, wherein the fluorescent detection agent comprises an affinity reagent attached to another structured nucleic acid particle, wherein the affinity reagent is bound to the biological entity.
Claims24
A composition, comprising:
- a. a substrate;
- b. a first structured nucleic acid particle coupled to the substrate, wherein a biological entity is coupled to the first structured nucleic acid particle;
- and
- c. a fluorescent detection agent, wherein the fluorescent detection agent comprises an affinity reagent attached to a second structured nucleic acid particle, wherein the affinity reagent is bound to the biological entity, wherein the first structured nucleic acid particle is coupled to the substrate by binding between a first oligonucleotide and a second oligonucleotide, wherein the first oligonucleotide is attached to the substrate, and wherein the second oligonucleotide comprises a strand of the structured nucleic acid particle.
Dependencies are inferred from the claim text.