Psicose-6-phosphate phosphatase, composition for producing psicose comprising the same, and method for producing psicose using the same
AssigneeCj Cheiljedang Corporation
InventorsHyun June Park, Sang Young Yoon, Hyun Kug Cho, Sung Jae Yang, So-hyeong Kim, Seung Hwan Kim, Il Hyang Park, Byung-sam Son, Seong Bo Kim, Seung Won Park
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Abstract
The present application relates to a psicose-6-phosphate phosphatase, a microorganism comprising the same, and a method for producing psicose using the same.
Claims20
A method for producing psicose, comprising converting psicose-6-phosphate into psicose by bringing psicose-6-phosphate into contact with a psicose-6-phosphate phosphatase, a microorganism expressing the same, or a culture of the microorganism, wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of the genus Alicyclobacillus, Amycolatopsis, Anaerolinea, Archaeoglobus, Bacillus, Caldicellulosiruptor, Caldilinea, Caldithrix, Carboxydocella, Carboxydothermus, Chloroflexi, Defluviitoga, Deinococcus, Desulfurococcus, Dictyoglomus, Effusibacillus, Fervidobacterium, Geobacillus, Halococcus, Hydrogenivirga, Hydrogenobacter, Hyperthermus, Kosmotoga, Marinitoga, Meiothermus, Mesotoga, Metallosphaera, Methanocella, Methanococcoides, Methanohalobium, Methanolobus, Methanosarcina, Methanothermus, Petrotoga, Picrophilus, Pseudonocardia, Pyrococcus, Pyrodictium, Rhodothermus, Slackia, Staphylothermus, Sulfolobus, Thermanaerothrix, Thermoanaerobacter, Thermoanaerobacterium, Thermobifida, Thermococcus, Thermocrinis, Thermoflexus, Thermotoga, Thermus, and Truepera.
A method for producing psicose, comprising bringing starch, maltodextrin, sucrose, or a combination thereof into contact with (a) a psicose-6-phosphate phosphatase;
- fructose-6-phosphate-3-epimerase;
- glucose-6-phosphate-isomerase;
- phosphoglucomutase or glucokinase;
- and α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, α-amylase, pullulanase, isoamylase, glucoamylase, α-glucanotransferase, polyphosphate glucokinase, or sucrase;
- or (b) a microorganism expressing the enzymes of item (a) or a culture of the microorganism, wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of the genus Alicyclobacillus, Amycolatopsis, Anaerolinea, Archaeoglobus, Bacillus, Caldicellulosiruptor, Caldilinea, Caldithrix, Carboxydocella, Carboxydothermus, Chloroflexi, Defluviitoga, Deinococcus, Desulfurococcus, Dictyoglomus, Effusibacillus, Fervidobacterium, Geobacillus, Halococcus, Hydrogenivirga, Hydrogenobacter, Hyperthermus, Kosmotoga, Marinitoga, Meiothermus, Mesotoga, Metallosphaera, Methanocella, Methanococcoides, Methanohalobium, Methanolobus, Methanosarcina, Methanothermus, Petrotoga, Picrophilus, Pseudonocardia, Pyrococcus, Pyrodictium, Rhodothermus, Slackia, Staphylothermus, Sulfolobus, Thermanaerothrix, Thermoanaerobacter, Thermoanaerobacterium, Thermobifida, Thermococcus, Thermocrinis, Thermoflexus, Thermotoga, Thermus, and Trueper.
A method for producing psicose, comprising converting psicose-6-phosphate into psicose by bringing psicose-6-phosphate into contact with a psicose-6-phosphate phosphatase, a microorganism expressing the same, or a culture of the microorganism,
- wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence having a sequence identity of at least 70% to any one of amino acid sequences of SEQ ID NOS:
- 1, 6, 9, 12 to 14, 17, 18, 20 to 23, 25, 26, 28, 29, 37 to 53, 55 to 57, 59 to 61, 64 to 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91 to 93, 95, 99 to 105, 108, 113, 114, 116, 117, 122, 131, 133, 134, 136, 142 to 148, 150 to 152, 154 to 156, 159 to 164, 167, 169, 172, 173, 175, 177, 184 to 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, and 221.
Dependencies are inferred from the claim text.