US 12,305,202 B2Granted

Polypeptide composition

AssigneesJecho Laboratories, Inc., Jecho Institute, Co., Ltd.

InventorsJianwei Zhu, Jing Wang, Lei Han, Junsheng Chen, Yueqing Xie, Hua Jiang

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Abstract

A polypeptide composition, and methods of making the same, may include a first polypeptide and a second polypeptide, wherein the first polypeptide comprise a first toxin fragment and a first intein fragment, the second polypeptide comprise a second toxin fragment and a second intein fragment, the first polypeptide is different from the second polypeptide; the first toxin fragment and the second toxin fragment are non-biotoxic; the first polypeptide and the second polypeptide may make the first toxin fragment and the second toxin fragment into biotoxic toxins by an interaction of the first intein fragment and the second intein fragment. Kits may include such first and second polypeptides, and such compositions and kits may be used in treating tumors.

Claims19

Dependent claims

A composition, comprising:

a first polypeptide;
and a second polypeptide, wherein the first polypeptide comprises a first toxin fragment and a first intein fragment, wherein the second polypeptide comprises a second toxin fragment and a second intein fragment, wherein the first polypeptide is different from the second polypeptide, wherein the first toxin fragment and the second toxin fragment are non-biotoxic, and wherein the first polypeptide and the second polypeptide are capable of making the first toxin fragment and the second toxin fragment to form a toxin with biotoxicity by an interaction between the first intein fragment and the second intein fragment, and wherein the toxin is PE38, which is a truncated form of exotoxin PE from Pseudomonas aeruginosa;
wherein the first toxin fragment consists of the amino acid sequence of SEQ ID NO:
4, and the second toxin fragment consists of the amino acid sequence of SEQ ID NO:
14.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/280,636Filed 27 Sep 2019
Priority: CN 201811162604.030 Sep 2018
Published: US 2022/0282231 A18 Sep 2022
Granted: 20 May 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification17 codes

C12N 9/1077
Glycosyltransferases: pentosyltransferases
C12N 9/14
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: hydrolases
A61P 35/00
Antineoplastic agents
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria