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US 12,305,197 B2Granted
Proliferation method
AssigneesKao Corporation, Shizuoka Prefectural University Corporation
InventorsShintaro Onishi, Takuya Mori, Takashi Suzuki, Yuuki Kurebayashi
Life of this patent
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Abstract
Provided is a method for efficiently proliferating an influenza virus serving as a material for vaccine in a host. A method for proliferating an influenza virus in a host, comprising a step of inhibiting transfer of Bax in a host cell to the inner mitochondrial membrane.
Claims9
A method for proliferating an influenza virus in a host, the method comprising the steps of:
- 1. Infecting the host with an influenza virus;
- 2. Culturing the influenza virus-infected host of Step 1 under conditions in which the influenza virus replicates;
- and
- 3. Adding a Bax inhibitor to the host, either before the infecting of Step 1, or after the infecting of Step 1, or simultaneously with the infecting of Step 1, wherein the Bax inhibitor inhibits transfer of Bax to the host's inner mitochondrial membrane;
- wherein the host is an embryonated chicken egg or a cultured cell that is susceptible to the influenza virus.
2 more elements
A method for preparing influenza virus particles, comprising the steps of:
- 1. Infecting the host with an influenza virus;
- 2. Culturing the influenza virus-infected host of Step 1 under conditions in which the influenza virus replicates;
- 3. Adding a Bax inhibitor to the host, either before the infecting of Step 1, or after the infecting of Step 1, or simultaneously with the infecting of Step 1, wherein the Bax inhibitor inhibits transfer of Bax to the host's inner mitochondrial membrane;
- and
- 4. Collecting influenza virus particles from the culture after completion of the culturing of Step 2;
- wherein the host is an embryonated chicken egg or a cultured cell that is susceptible to the influenza virus.
3 more elements
Dependencies are inferred from the claim text.