Method for producing artificial recombinant RNA virus stably retaining foreign gene
AssigneeOsaka University
InventorsTakeshi Kobayashi, Yuta Kanai
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Abstract
Provided is a method for producing an artificial recombinant RNA virus stably expressing a foreign gene, comprising the steps of: (1) obtaining a foreign gene having a modified codon composition similar to that of an RNA virus gene; (2) inserting the foreign gene obtained in step (1) into an RNA virus genome; and (3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics.
Claims4
A method for producing an artificial recombinant RNA virus stably retaining a foreign gene, comprising the steps of:
- (1) obtaining a foreign gene having a codon composition modified to such an extent that the difference in the codon composition between the foreign gene and an RNA virus gene is within +30%;
- (2) inserting the foreign gene obtained in step (1) into an RNA virus genome;
- and
- (3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics, wherein the RNA virus is a virus belonging to the family Reoviridae.
A method for enabling stable retention of an introduced foreign gene in an artificial recombinant RNA virus artificially synthesized using reverse genetics, comprising modifying the codon composition of a foreign gene to such an extent that the difference in the codon composition between the foreign gene and an RNA virus gene is within +30%, wherein the RNA virus is a virus belonging to the family Reoviridae.
Dependencies are inferred from the claim text.