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US 12,305,185 B2Granted
Expression vector and method
AssigneeMount Spec Investments Pty Ltd
InventorsChristopher Layton, Jason Steel
Life of this patent
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Abstract
The present disclosure relates to regulatable expression vectors for systematically controlling and silencing expression of a therapeutic molecule and uses thereof, e.g. treating ocular disorders such as choroidal neovascularisation.
Claims10
An expression vector comprising
- a) a kill switch comprising a first site-specific recombination sequence and a second site-specific recombination sequence;
- b) a regulatable element operably linked to a nucleic acid sequence encoding a therapeutic molecule, wherein activity of the regulatable element is regulated by a regulator compound;
- and
- c) a constitutive promoter operably linked to a nucleic acid sequence encoding a regulator compound-binding polypeptide which is capable of binding a regulator compound, wherein upon binding the regulator compound, the regulator compound-binding polypeptide regulates expression of the therapeutic molecule,
- wherein activation of the kill switch by recombination between the first site-specific recombination sequence and the second site-specific recombination sequence silences expression of the nucleic acid encoding the therapeutic molecule from the vector.
2 more elements
Dependencies are inferred from the claim text.