System and method for centralized fluid management and culture control

A method for automatic centralized fluid management and culture control comprising:

• (a) automatically, by a system controller, monitoring temperature, nutrient concentration, and dissolved gas concentrations of a cell or tissue culture media;
• (b) automatically, by the system controller, maintaining the temperature of the cell or tissue culture media in a media reservoir at a first pre-selected value;
• (c) automatically, by the system controller, adjusting the temperature, the nutrient concentration, and the dissolved gas concentrations of the cell or tissue culture media to second pre-selected values prior to delivery to a user-supplied device;
• (d) automatically, by the system controller, delivering the temperature, the nutrient concentration, and dissolved gas concentration-adjusted cell or tissue culture media to the user-supplied device;
• (e) automatically, by the system controller, recirculating the temperature, the nutrient concentration, and the dissolved gas concentration-adjusted cell or tissue culture media through a flow path including the media reservoir, a reservoir line, a reservoir module cassette, a holding container, a module line, and a bioreactor module cassette within the user-supplied device;
• (f) automatically, by the system controller, monitoring the temperature, the nutrient concentration, and the dissolved gas concentrations of the adjusted cell or tissue culture media to verify they remain at the second pre-selected values through the user-supplied device;
• (g) if the temperature, or the nutrient concentration, or the dissolved gas concentrations of the adjusted cell or tissue culture media meet the second pre-selected values, automatically, by the system controller, repeating steps (e)-(f);
• and
• (h) if the temperature, or the nutrient concentration, or the dissolved gas concentrations of the adjusted cell or tissue culture media do not meet the second pre-selected values, automatically, by the system controller, delivering the recirculated adjusted cell or tissue culture media to a waste vessel or a sample vessel.