Process for microbial synthesis and an apparatus thereof
AssigneeIndian Oil Corporation Limited
InventorsPrakash Chandra Sahoo, Manoj Kumar, Ravi Prakash Gupta, Suresh Kumar Puri, Deepak Saxena, Sankara Sri Venkata Ramakumar
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Abstract
The present invention relates to a process for microbial synthesis process having less water consumption and yielding desired product profile. More particularly, the present invention relates to an improved process of microbial synthesis using ultra fine nutrient mist in a specially designed biofilm-bioreactor under controlled conditions. The present invention also relates to an apparatus for microbial synthesis and preparation of optimized biofilm for continuous product formation.
Claims17
A process for microbial synthesis, the process comprising:
- diluting a pre grown inoculum with saline water having ZnSe quantum dots, and a silica gel mixture to prepare an inoculum mixture, wherein the pre grown inoculum comprises microbes, wherein the microbes are selected from the group consisting of Enterobacter aerogenes MTCC 25016, Serratia sp. MTCC 25017, Shewanella sp. MTCC 25020 and Alcaligenes sp. MTCC 25022;
- spraying the inoculum mixture on a biofilm support placed in a mist bioreactor to form a biofilm;
- adding a nutrient mist medium, a homoserine lactone, and dioleoylphosphatydic acid and mechanically stirring for about 30 minutes to form a homogeneous mixture;
- sending the homogeneous mixture to a mist generation chamber to form an ultra-fine mist, wherein the ultra-fine mist is formed by ultra sonication;
- passing the ultra-fine mist through a UV chamber for a duration of 2-5 seconds;
- sparging nitrogen gas along with the ultra-fine mist into the mist bioreactor;
- wherein the ratio of the ultra-fine mist and the nitrogen gas is 1:2;
- passing a feed through the mist bioreactor having the biofilm and collecting a product in a product collection and separation chamber.
Dependencies are inferred from the claim text.