Hyaluronic acid-collagen copolymer compositions and medical applications thereof
AssigneeShanghai Qisheng Biological Preparation Co., Ltd.
InventorsDale P. DeVore, Jiaxun Zhu
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Abstract
The present disclosure relates to glycosaminoglycan-collagen copolymer compositions (such as hyaluronic acid-collagen copolymers and heparosan-collagen copolymer compositions) and medical applications thereof for augmenting soft tissue defects. The copolymer composition may be injected into tissues to correct defects or deficiencies, such as skin wrinkles, scars, and folds in dermal tissues.
Claims21
A method for preparing a glycosaminoglycan-soluble collagen copolymer comprising the steps of:
- a) chemically grafting poly-L-lysine (PLL) to the glycosaminoglycan by reacting the glycosaminoglycan with PLL to produce a glycosaminoglycan-PLL gel;
- b) chemically derivatizing soluble collagen by reacting a collagen solution at an alkaline pH with an acetylation agent to produce a collagen gel derivative;
- c) mixing the glycosaminoglycan-PLL gel with the collagen gel derivative;
- and
- d) adding a bifunctional acetylation agent to react with free amine groups on glycosaminoglycan-PLL and collagen gel derivative to produce a mixture of glycosaminoglycan-PLL-collagen gel, glycosaminoglycan-PLL polymer, and collagen gel derivative polymer,
- wherein the glycosaminoglycan is selected from hyaluronic acid, heparosan, a salt of hyaluronic acid, a salt of heparosan, and combinations thereof.
A method for preparing a hyaluronic acid-soluble collagen copolymer comprising the steps of:
- a) chemically grafting poly-L-lysine (PLL) to hyaluronic acid by reacting the hyaluronic acid with PLL to produce a hyaluronic acid-PLL gel;
- b) chemically derivatizing soluble collagen by reacting a collagen solution at an alkaline pH with an acetylation agent to produce a collagen gel derivative;
- c) mixing the hyaluronic acid-PLL gel with the collagen gel derivative;
- and
- d) adding a bifunctional acetylation agent to react with free amine groups on hyaluronic acid-PLL and collagen gel derivative to produce a mixture of hyaluronic acid-PLL-collagen gel, hyaluronic acid-PLL polymer, and collagen gel derivative polymer.
A method for preparing a hyaluronic acid-soluble collagen copolymer comprising the steps of:
- a) chemically grafting poly-L-lysine (PLL) to hyaluronic acid by reacting the hyaluronic acid with PLL to produce a hyaluronic acid-PLL gel;
- b) chemically derivatizing soluble collagen by reacting a collagen solution at an alkaline pH with an acetylation agent to produce a collagen gel derivative;
- c) mixing the hyaluronic acid-PLL gel with the collagen gel derivative;
- and
- d) adding a bifunctional acetylation agent to react with free amine groups on hyaluronic acid-PLL and collagen gel derivative to produce a mixture of hyaluronic acid-PLL-collagen gel, hyaluronic acid-PLL polymer, and collagen gel derivative polymer,
- wherein the hyaluronic acid is a metallic salt of hyaluronic acid.
Dependencies are inferred from the claim text.