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US 12,304,932 B2Granted
PACAP stabilized peptide
AssigneeSenju Pharmaceutical Co., Ltd.
InventorsShinnosuke Machida, Takeshi Nakajima
Life of this patent
Estimating the term…
Abstract
The purpose of the present invention is to provide a PACAP peptide having increased stability. The problem is solved by the discovery that substituting a carboxy group of the position 3 and/or position 8 aspartic acid in the PACAP sequence by tetrazole significantly enhances stability.
Claims6
A peptide consisting of a sequence or modified sequence wherein, in the sequence represented by the following formula:
- (SEQ ID NO:
- 3)
- H-X1-D-G-I-F-T-D-X2-Y-X3-R-Y-R-X4-X5-X6-A-X7-X8-
- X9-Y-L-A-A-V-X10
- wherein:
- X1, X2 and X3 are alanine or serine,
- X4, X8 and X9 are lysine or arginine,
- X5 is glutamine, alanine or an amino acid having the amide of glutamine replaced with tetrazole,
- X6 is methionine, norleucine, alanine or leucine,
- X7 is valine or alanine, and
- X10 is leucine or alanine,
- wherein the carboxy group of the aspartic acid residue at position 3 and/or position 8 is replaced with tetrazole, and optionally one, two or three amino acids is deleted from C-terminus of the sequence of SEQ ID NO:
- 3, or any one sequence selected from the group consisting of:
- (SEQ ID NO:
- 37)
- GKRYKQRVKNK;
- (SEQ ID NO:
- 38)
- GKRYKQRVKN;
- (SEQ ID NO:
- 39)
- GKRYKQRVK;
- (SEQ ID NO:
- 40)
- GKRYKQRV;
- (SEQ ID NO:
- 41)
- GKRYKQR;
- (SEQ ID NO:
- 42)
- GKRYKQ;
- (SEQ ID NO:
- 43)
- GKRYK;
- (SEQ ID NO:
- 44)
- GKRY
- GKR;
- GRR;
- GK;
- and
- GR
- G;
- is added at C-terminus of the sequence of SEQ ID NO:
- 3.
42 more elements
Dependencies are inferred from the claim text.