Methods and materials for using fibrin supports for retinal pigment epithelium transplantation
AssigneeMayo Foundation for Medical Education and Research
InventorsAlan D. Marmorstein, Raymond Iezzi, Jarel K. Gandhi, Jose S. Pulido
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Abstract
This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.
Claims23
A retinal implant comprising (a) a retinal pigment epithelium monolayer having an apical surface and a basal surface, and (b) a fibrin hydrogel layer directly attached to said basal surface of said monolayer by a process comprising the presence of a protease inhibitor or an anti-fibrinolytic agent, wherein said apical surface is free of a fibrin hydrogel layer, wherein said retinal implant lacks patches of degraded fibrin hydrogel within said fibrin hydrogel layer, and wherein said fibrin hydrogel layer degrades when said retinal implant is implanted into an eye of a mammal.
A method for making a retinal implant, wherein said method comprises culturing retinal epithelial cells directly on a fibrin basal support substrate in a medium comprising a protease inhibitor or an anti-fibrinolytic agent to form a retinal pigment epithelium monolayer having an apical surface and a basal surface, wherein said basal surface is closer to said fibrin basal support substrate than said apical surface, wherein said apical surface is free of said fibrin basal support substrate, wherein said retinal implant lacks patches of degraded fibrin hydrogel within said fibrin hydrogel layer, and wherein said fibrin hydrogel layer degrades when said retinal implant is implanted into an eye of a mammal.
Dependencies are inferred from the claim text.