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US 12,303,615 B2Granted
Demineralized bone paper
AssigneeUniversity of Massachusetts
InventorsJungwoo Lee, Jaehyuck Shim, Ryan Carpenter, Yongkuk Park, Jun-Goo Kwak
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Abstract
Disclosed herein are three-dimensional bone tissue grafts produced from stacked demineralized bone paper. Also disclosed are methods for treating a subject using tissue grafts produced from the disclosed demineralized bone paper. Also disclosed are assay systems that involves culturing bone-promoting cells on the disclosed demineralized bone paper.
Claims6
A method for assaying the effect of an agent on bone development or health, comprising
- (a) providing an assay system comprising
- (i) a multi-well culture plate,
- (ii) one or more layers of semi-transparent, demineralized, compact bone slices 10-1,000 μm thick in one or more wells of the multi-well culture plate, further comprising bone-promoting cells on the one or more layers, wherein the compact bone slices have been remineralized by the bone-promoting cells, and
- (iii) a culture medium configured to promote or maintain bone development;
- (b) adding a candidate agent to the culture medium of the assay system;
- and
- (c) measuring at least one parameter of bone development in the assay system to determine if the candidate agent affected bone development, bone resorption, or health.
4 more elements
Dependencies are inferred from the claim text.