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US 12,303,560 B2Granted
Recombinant bovine immunodeficiency virus-like particles comprising an influenza HA transmembrane domain and C-terminus
AssigneeMedigen, Inc.
InventorsPeter Pushko, Irina Tretyakova
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Abstract
Described herein are Bovine immunodeficiency virus gag protein (“Bgag”) recombinant virus like particles (“VLPs”) comprising one or more different types of target pathogen proteins. Also described, are compositions comprising the novel Bgag VLPs and the methods of making and using the novel Bgag VLPs.
Claims30
A bovine immunodeficiency virus gag virus like particle (“Bgag VLP”) comprising
- an Influenza HA transmembrane domain and C Terminus (“TMCT”) and
- a heterologous antigen,
- wherein the heterologous antigen is functionally connected to the HA TMCT.
A method of making a bovine immunodeficiency virus gag virus like particle (“Bgag VLP”), comprising of the steps:
- (i) cloning a transfer vector plasmid comprising:
- a bovine immunodeficiency virus gag (“Bgag”) gene;
- one or more genes encoding an Influenza HA transmembrane domain and C Terminus (“TMCT”);
- and
- one or more genes encoding a heterologous antigen;
- and
- one or more promoter;
- wherein the promoter is operably linked to the Bgag gene and the one or more genes encoding the TMCT;
- (ii) preparing a carrier virus using the transfer vector plasmid;
- and
- (iii) infecting eukaryotic cells to produce the Bgag VLP;
- wherein the carrier virus is prepared in the absence of a second transfer vector plasmid.
9 more elements
Dependencies are inferred from the claim text.