Multivalent Epstein-Barr virus-like particles and uses thereof
AssigneeCity of Hope
InventorsJavier Gordon Ogembo, Lorraine Zvichapera Mutsvunguma, Felix Wussow
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Abstract
Disclosed are vaccine compositions comprising a VLP comprising two or more EBV envelope glycoproteins and one or more T cell antigens and methods of preventing or treating EBV infections using the vaccine compositions. Also disclosed is an expression system or a single expression vector for co-expressing two or more EBV envelope glycoproteins simultaneously to generate a VLP vaccine. The expression system may include a single vector inserted with two or more nucleic acid sequences that encode two or more EBV envelope glycoproteins linked by one or more linking sequences such that the EBV envelope glycoproteins are co-expressed simultaneously.
Claims20
An expression system for co-expressing a plurality of EBV envelope glycoproteins comprising an expression vector having two or more expression cassettes, wherein each of the two or more expression cassettes comprises:
- a promoter,
- a nucleic acid sequence encoding two or more EBV envelope glycoproteins, and
- one or more linking sequences;
- wherein the two or more EBV envelope glycoproteins are co-expressed simultaneously, self-cleaved and/or self-processed to assemble into one or more glycoprotein complexes.
A composition comprising an expression vector having two or more expression cassettes, wherein each of the two or more expression cassettes comprises:
- a promoter,
- a nucleic acid sequence encoding two or more EBV envelope glycoproteins, and
- one or more linking sequences;
- wherein the two or more EBV envelope glycoproteins can are co-expressed simultaneously, self-cleaved and/or self-processed to assemble into one or more glycoprotein complexes;
- and
- wherein the expression vector is capable of co-expressing a plurality of EBV envelope glycoproteins.
Dependencies are inferred from the claim text.