Simplification of method or system using scout MRM
AssigneeDH Technologies Development Pte. Ltd.
InventorsDavid M. Cox, Chang Liu
Life of this patent
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Abstract
Each sample of a series of samples is ejected at an ejection time and according to a sample order. Each ejected sample of the series is ionized, producing ion beam. A list of different sets of MRM transitions is received. Each set of the list corresponds to a different sample. A group of one or more different sets is selected from the list. Initially, each set selected for the group corresponds to a different sample of one or more first samples of the series. A mass spectrometer is instructed to execute each transition of each set of the group on the ion beam until a transition of a set of the group is detected, upon which, one or more next sets are selected from the list to be monitored using the set of the detected transition and the sample order.
Claims15
A system for selecting the next multiple reaction monitoring (MRM) transitions to be monitored in a high-throughput sample introduction coupled mass spectrometry experiment based on the transition detected and the order of the samples, comprising:
- a sample introduction system that ejects each sample of a series of samples at an ejection time and according to a sample order, producing a plurality of ejection times corresponding to the series, and ionizes each ejected sample of the series, producing an ion beam;
- a tandem mass spectrometer that receives the ion beam;
- and
- a processor that
- receives a list of different sets of one or more MRM precursor ion to product ion transitions, wherein each set of the list corresponds to a different sample of the series,
- selects a group of one or more sets from the list, wherein initially each of one or more sets selected for the group corresponds to a different sample of one or more first samples of the series,
- instructs the tandem mass spectrometer to execute each transition of each set of the group on the ion beam during each cycle of a plurality of cycles until a transition of a set of the group is detected, and
- when a transition of a set of the group is detected, selects one or more next sets from the list to be monitored using the detected transition and the sample order.
A method for selecting the next multiple reaction monitoring (MRM) transitions to be monitored in a high-throughput sample introduction coupled mass spectrometry experiment based on the transition detected and the order of the samples, comprising:
- ejecting each sample of a series of samples at an ejection time and according to a sample order, producing a plurality of ejection times corresponding to the series, and ionizing each ejected sample of the series, producing an ion beam, using a sample introduction system;
- receiving the ion beam using a tandem mass spectrometer;
- receiving a list of different sets of one or more MRM precursor ion to product ion transitions using a processor, wherein each set of the list corresponds to a different sample of the series;
- selecting a group of one or more sets from the list using the processor, wherein initially each of one or more sets selected for the group corresponds to a different sample of one or more first samples of the series;
- instructing the tandem mass spectrometer to execute each transition of each set of the group on the ion beam during each cycle of a plurality of cycles until a transition of a set of the group is detected using the processor;
- and
- when a transition of a set of the group is detected, selecting one or more next sets from the list to be monitored using the detected transition and the sample order using the processor.
A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for selecting the next multiple reaction monitoring (MRM) transitions to be monitored in a high-throughput sample introduction coupled mass spectrometry experiment based on the transition detected and the order of the samples, the method comprising:
- providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a control module;
- instructing a sample introduction system to eject each sample of a series of samples at an ejection time and according to a sample order, producing a plurality of ejection times corresponding to the series, and ionize each ejected sample of the series, producing an ion beam, using the control module;
- instructing a tandem mass spectrometer to receive the ion beam using the control module;
- receiving a list of different sets of one or more MRM precursor ion to product ion transitions using the control module, wherein each set of the list corresponds to a different sample of the series;
- selecting a group of one or more sets from the list using the control module, wherein initially each of one or more sets selected for the group corresponds to a different sample of one or more first samples of the series;
- instructing the tandem mass spectrometer to execute each transition of each set of the group on the ion beam during each cycle of a plurality of cycles until a transition of a set of the group is detected using the control module;
- and
- when a transition of a set of the group is detected, selecting one or more next sets from the list to be monitored using the detected transition and the sample order using the control module.
Dependencies are inferred from the claim text.