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US 12,298,211 B2Granted
Device for tissue section immobilization and retention
AssigneeSingular Genomics Systems, Inc.
InventorsYuji Ishitsuka, Zhenmin Hong, Eli N. Glezer, Hu Cang, William Dempsey, Weiqiao Ding, Jaekyung Koh, Mohammad Vatankhah Varnosfaderani
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Abstract
Disclosed herein, inter alia, are devices and methods for transfer and analysis of tissue sections using carrier substrates.
Claims19
A method of detecting nucleic acids in a tissue section, comprising:
- contacting a plurality of discrete tissue samples attached to a solid support with a plurality of circularizable probes, wherein a circularizable probe binds to a nucleic acid molecule in a discrete tissue sample, and wherein each discrete tissue sample comprises a tissue section comprising a width of about 4 mm to about 10 mm and a thickness of about 5 μm to about 12 μm;
- forming a circular oligonucleotide in the tissue section, and amplifying the circular oligonucleotide to form amplification products;
- and
- detecting the amplification products by binding a fluorescently labeled probe to the amplification product and detecting the fluorescent label.
1 more element
Dependencies are inferred from the claim text.