Preparation of tissue sections using fluorescence-based detection
AssigneeAgilent Technologies, Inc.
InventorsKyle Schleifer, Kristin Briana Bernick, Adrienne Mccampbell, Nicholas M. Sampas, Victor Lim
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Abstract
The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
Claims19
A method to distinguish tissue from embedding medium, the method comprising:
- irradiating an embedded tissue sample with light having a wavelength of from about 200 nm to about 600 nm, wherein the embedded tissue sample comprises a tissue and an embedding medium, and the tissue is optically indistinguishable from the embedding medium under normal lighting conditions;
- and
- analyzing endogenous fluorescence emission of the embedded tissue sample.
A method to distinguish tissue from embedding medium in an embedded tissue sample, the method comprising:
- providing an embedded tissue sample comprising a tissue and an embedding medium,
- irradiating the embedded tissue sample with light having a wavelength of from about 200 nm to about 600 nm, and
- analyzing endogenous fluorescence emission of the embedding medium.
Dependencies are inferred from the claim text.