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US 12,297,487 B2Granted
Spatially encoded biological assays
AssigneePrognosys Biosciences, Inc.
InventorMark S. Chee
Life of this patent
Estimating the term…
Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
Claims29
A composition comprising:
- (i) a substrate and a plurality of capture probes, and
- (ii) a tissue section, wherein the tissue section is disposed on the substrate, and each of the plurality of capture probes comprises:
- (a) a target-binding domain that comprises a nucleic acid sequence that is configured to bind to at least a portion of a nucleic acid that binds to the target- binding domain;
- (b) a nucleic acid sequence that identifies a location on the substrate and is not configured to hybridize to the nucleic acid that binds to the target-binding domain, and
- (c) a primer binding site.
2 more elements
A composition for spatial analysis comprising:
- (i) a flow cell comprising a substrate having a plurality of wells, and
- (ii) a plurality of capture probes immobilized in the wells of the substrate, wherein each of the plurality of capture probes comprises:
- (a) a target-binding domain that comprises a nucleic acid sequence that is configured to bind to at least a portion of a nucleic acid that binds to the target-binding domain;
- (b) a nucleic acid sequence that identifies a location of the capture probe on the substrate and is not configured to hybridize to the nucleic acid that binds to the target-binding domain, and
- (c) a primer binding site.
2 more elements
Dependencies are inferred from the claim text.