Rapid cell culture test device designed such that fluid films with uniform thickness are formed

A cell analysis method using a multi-well-based cell culture test device,

• wherein,
• the multi-well-based cell culture test device comprises an array structure of a plurality of aligned well units,
• each of the plurality of aligned well units comprises a first sub-well in which a first fluid is accommodated and a barrier structure surrounding the first sub-well to define the area of the first sub-well,
• a capillary channel recessed from the bottom surface of the first sub-well while traversing the bottom surface is formed to accommodate the first fluid,
• the barrier structure is divided into a barrier portion A located adjacent to one end of the capillary channel and a barrier portion B located adjacent to the other end of the capillary channel, and
• the barrier portion A being greater in height than the barrier portion B such that when the barrier portion A is wetted with the first fluid introduced into the first sub-well along its inner wall, a change in the level of the first fluid at the other end of the capillary channel depending on the amount of the first fluid dispensed is minimized,
• the method comprises:
• (a) providing a mixture solution of a gelling agent-containing liquid medium and a biological agent to the capillary channel recessed from the bottom surface of the first sub-well,
• (b) gelling the mixture solution to form a solid thin film that is immobilized with the biological agent and minimizes elevation of the liquid-gas interface,
• (c) providing a solution containing a bioactive agent to a second sub-well or providing a solvent capable of dissolving a bioactive agent in a solid form to a second sub-well loaded with the bioactive agent,
• (d) introducing the bioactive agent-containing solution or the solvent capable of dissolving the bioactive agent in such an amount that the solution or the solvent crosses over the barrier, to allow the bioactive agent to diffuse into the solid thin film in the first sub-well, and
• (e) observing responses of the biological agent to the bioactive agent.