Lipid-encapsulated dual-cleaving endonuclease for DNA and gene editing
AssigneeSpecific Biologics Inc.
InventorsDavid R. Edgell, Thomas A. McMurrough, Brent E. Stead, Odisho K. Israel
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Abstract
Methods to edit genes by administering a chimeric nuclease to a cell or organism without the use of a viral vector.
Claims18
A nuclease comprising a modified Staphylococcus aureus Cas9, wherein said modified Staphylococcus aureus Cas9 comprises an aspartic acid to glutamic acid substitution at an amino acid corresponding to position 10 of SEQ ID NO:
- 13, and wherein the amino acid sequence of the Staphylococcus aureus Cas9 has at least 99% sequence identity to SEQ ID NO:
- 13.
A chimeric nuclease comprising an I-Tevl nuclease domain, a linker, a Staphylococcus aureus Cas9, and a guide RNA, wherein the Staphylococcus aureus Cas9 comprises an aspartic acid to glutamic acid substitution at an amino acid corresponding to position 10 of SEQ ID NO:
- 13, and wherein the amino acid sequence of the Staphylococcus aureus Cas9 has at least 99% sequence identity to SEQ ID NO:
- 13.
Dependencies are inferred from the claim text.